| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rhizobium leguminosarum | TACTGCGCAACCACGGTT | TTCCTCCAGAACGGCACTG | 59.89 | 59.63 | 200 | 91 |
100.00%
|
50.00%
|
| Primer 2 | Rhizobium leguminosarum | TGATGATCGCACACGGCA | TCTCGTGTTCGGAATGGCC | 59.74 | 60.08 | 294 | 84 |
100.00%
|
100.00%
|
| Primer 3 | Rhizobium leguminosarum | TGATGATCGCACACGGCA | CCCTCCAGCTTTACGGCAT | 59.74 | 59.78 | 160 | 84 |
100.00%
|
50.00%
|
| Primer 4 | Rhizobium leguminosarum | TGATGATCGCACACGGCA | CAGCTTTACGGCATCGCAG | 59.74 | 59.65 | 155 | 84 |
100.00%
|
100.00%
|
| Primer 5 | Rhizobium leguminosarum | TGATGATCGCACACGGCA | CAGCTTTACGGCATCGCA | 59.74 | 58.51 | 155 | 84 |
100.00%
|
50.00%
|
| Primer 6 | Rhizobium leguminosarum | TTGCCGATCTGCGCAGAA | AAGTGCGGTGCCCATCAA | 60.05 | 59.88 | 179 | 82 |
100.00%
|
100.00%
|
| Primer 7 | Rhizobium leguminosarum | TTGCCGATCTGCGCAGAA | GGAAGGCTGGGCTCGAAAT | 60.05 | 60.08 | 297 | 82 |
100.00%
|
100.00%
|
| Primer 8 | Rhizobium leguminosarum | TTGCCGATCTGCGCAGAA | CGACGATGGCAAAGGCAAG | 60.05 | 59.86 | 195 | 82 |
100.00%
|
100.00%
|
| Primer 9 | Rhizobium leguminosarum | TTGCCGATCTGCGCAGAA | GGAGAGATCGGCAAGCGAA | 60.05 | 59.86 | 254 | 82 |
100.00%
|
100.00%
|
| Primer 10 | Rhizobium leguminosarum | TTGCCGATCTGCGCAGAA | ATGGCAAAGGCAAGTGCG | 60.05 | 59.35 | 190 | 78 |
100.00%
|
100.00%
|